(Flow cytometry, FCM) ke sehlahlo sa lisele se lekanyang matla a fluorescence a matšoao a lisele tse silafetseng. Ke thekenoloji ea theknoloji e phahameng e entsoeng ho latela tlhahlobo le ho hlopha lisele tse le 'ngoe. E ka lekanya kapele le ho arola boholo, sebopeho sa ka hare, DNA, RNA, liprotheine, li-antigen le thepa e meng ea 'mele kapa ea lik'hemik'hale ea lisele,' me e ka ipapisa le pokello ea lihlopha tsena.
Flow cytometer haholo-holo e na le likarolo tse hlano tse latelang:
1 Phallo ea kamore le tsamaiso ea fluidics
2 Mohloli oa leseli la laser le sistimi ea ho bopa beam
3 Sistimi ea optical
4 Sistimi ea elektronike, polokelo, pontšo le tlhahlobo
5 Sistimi ea ho hlopha lisele
Har'a tsona, ho hlasimoloha ha laser mohloling oa lebone la laser le sistimi ea ho bopa marako ke tekanyo ea mantlha ea matšoao a fluorescence ho cytometry ea phallo. Matla a lebone la ho hlasimolla le nako ea ho pepeseha li amana le matla a lets'oao la fluorescence. Laser ke mohloli o lumellanang oa leseli o ka fanang ka leseli le leqhubu le le leng, matla a phahameng, le khanya e phahameng. Ke mohloli o loketseng oa khanya oa ho hlasimolla ho fihlela litlhoko tsena.
Ho na le lilense tse peli tsa cylindrical pakeng tsa mohloli oa laser le phaposi ea phallo. Lilense tsena li shebana le lebone la laser le nang le karolo e chitja e tsoang mohloling oa laser ho ea lebaleng la elliptical le karolo e nyane ea sefapano (22 μm × 66 μm). Matla a laser ka har'a libalaka lena la elliptical a ajoa ho latela kabo e tloaelehileng, ho netefatsa matla a khanya a tsitsitseng bakeng sa lisele tse fetang sebakeng sa ho bona laser. Ka lehlakoreng le leng, tsamaiso ea optical e na le lihlopha tse ngata tsa lilense, li-pinholes, le li-filters, tse ka arohanngoa ka lihlopha tse peli: holimo le tlaase ho phaposi ea phallo.
Sistimi ea optical ka pel'a kamore ea phallo e na le lense le pinhole. Mosebetsi o ka sehloohong oa lense le pinhole (hangata lilense tse peli le sekoti) ke ho shebana le lebone la laser le nang le sepakapaka se chitja se hlahisoang ke mohloli oa laser ho lebaleng la elliptical le karolo e nyane ea sefapano. Sena se aba matla a laser ho ea ka kabo e tloaelehileng, ho netefatsa matla a khanya a tsitsitseng bakeng sa lisele ho pholletsa le sebaka sa laser le ho fokotsa tšitiso ea leseli le lahlehileng.
Ho na le mefuta e meraro ea mantlha ea li-filters:
1: Sefate sa nako e telele (LPF) - se lumella feela leseli le nang le maqhubu a phahameng ho feta boleng bo itseng ho feta.
2: Short-pass filter (SPF) - e lumella feela khanya e nang le maqhubu a tlase ho boleng bo itseng hore e fete.
3: Filter ea Bandpass (BPF) - e lumella feela hore khanya e fete sebakeng se itseng sa maqhubu.
Metsoako e fapaneng ea li-filters e ka tsamaisa matšoao a fluorescence ka bolelele bo fapaneng ba maqhubu ho li-tubes tsa photomultiplier (PMTs). Mohlala, lihloela tsa ho bona fluorescence e tala (FITC) ka pele ho PMT ke LPF550 le BPF525. Lisefe tse sebelisoang ho bona orange-red fluorescence (PE) ka pele ho PMT ke LPF600 le BPF575. Lisefe tsa ho lemoha fluorescence e khubelu (CY5) ka pel'a PMT ke LPF650 le BPF675.
Flow cytometry e sebelisoa haholo-holo bakeng sa ho hlopha lisele. Ka tsoelo-pele ea mahlale a khomphutha, nts'etsopele ea immunology le ho qaptjoa ha thekenoloji ea antibody ea monoclonal, ts'ebeliso ea eona ho biology, bongaka, pharmacy le mafapha a mang a ntse a ata haholo. Lisebelisoa tsena li kenyelletsa tlhahlobo ea matla a lisele, apoptosis ea lisele, typing ea lisele, tlhahlobo ea tumor, tlhahlobo ea katleho ea lithethefatsi, jj.
Nako ea poso: Sep-21-2023
