(Phallo ea cytometry, fcm) ke sebui sa sele se lekanyang botebo ba matšoao a lisele. Ke se ngotsoeng ka mokhoa o phahameng ho ipapisitsoe le tlhahlobo le ho hlophisa lisele tsa masoha. E ka lekanya kapele le ho khetholla ka hare, sebopeho sa kahare, DNA, RNA, li-stereine tsa lisele, 'me li ka ipapisa le pokello ea lisele tsena.
Phallo ea cytometer e na le likarolo tse hlano tse latelang:
1 phallo ea phallo ea phaposi le speridis
2 Mohloli oa 2 oa lebone le beam shape system
Sistimi e 3 ea mahlo
4 Lihlopheng, polokelo, pontsho le tsamaiso ea tlhahlobo
5 Sistimi ea Mefuta ea Mefuta ea Sebele
Har'a bona, lase ho theosa mohlolineng oa laser lebone la laer le beam ke tekanyo ea mantlha ea lipontšo tsa fluometry. Botebo ba khanya ea mahlaseli le nako ea ho pepeseha li amana le botebo ba letšoao le ferekane. Laser ke mohloli o mong o kopaneng o ka fanang ka 'mala o le mong, boholo bo phahameng le ho hlatsuoa ho matla. Ke mohloli oa leseli o loketseng ho fihlela litlhoko tsena.
Ho na le lilebelo tse peli tsa cylindrical lipakeng tsa Mohloli oa Laser mohloli oa lipalesa. Lilense tsena li tsepamisa mohopolo oa laser ka karolo e chitja e tsoang mohloling oa laser se ka sebata se nang le skass-karolo ea 22 μm Ho latela moea oa laser ka har'a sebata sena sa elliptical ho tsamaisoa ho latela kabo e tloaelehileng, e netefatsa hore e tla hlakoloa ke lisele tse fetang sebakeng sa laser. Ka lehlakoreng le leng, tsamaiso ea mahlo e na le likarolo tse ngata tsa lilense, li-helhole le lifilimi tse ka arotsoeng ka lihlopha tse peli: holimo mokatong oa phallo.
Tsamaiso ea mahlo ka pel'a kamore ea phallo e na le lense le penhole. Ts'ebetso ea mantlha ea lense le lilense (hangata lilense (hangata lilense) ke ho tsepamisa likelello tsa laser ka moferefere oa Elliptical karolong e nyane ea mohala. Sena se fana ka matla a lasara ho ea ka kabo e tloaelehileng, e netefatsa hore ho hlakoloa lisele ho tsoa sebakeng sa tlhaselo ea laser le khanya e khelohileng.
Ho na le mefuta e meraro e meholo ea lifilimi:
1: Filter Filter (LPF) - e lumella feela leseli le phahamisang ho feta boleng bo ikhethileng ba ho feta.
2: Filter e khuts'oane (SPF) - e lumella leseli feela ka maratsoana a ka tlase ho boleng bo itseng ba ho feta.
3: Seteta sa bandpass (BPF) - e lumella leseli ho suglength e itseng ho feta.
Mefuta e fapaneng ea li-FALTER e ka tsamaisa lipontšo tse ka u thusang ho li-havelwhengthe tse fapaneng ho li-tubes tsa motho ka mong (PMTS). Mohlala, li-filthara bakeng sa ho lemoha Fluorescence e botala (Fitc) ka pele ho PMT ke LPF550 le BPF525. Lifilimi tse sebelisoang ho bona fluorescence e khubelu ea Orange (pe) ka pel'a PMT ke LPF600 le BPF575. Filters bakeng sa ho bona fluorescence e khubelu (cy5) ka pele ho PMT ke LPF650 le BPF675.
Ho phalla cytometry e sebelisoa haholo bakeng sa ho hlophisa sele. Ka tsoelo-pele ea mahlale a khomphutha, nts'etsopele ea mahlale a khokhopo, likopo tsa eona ho biology, meriana le masimo a mang a thatafala. Litheko tsena li kenyelletsa ho sekaseka lenane la lisele, Apoptosis ea sele, sele ea sele ea ho fumana tlhahlobo, ho sekaseka kalafo ea lithethefatsi, jj.
Nako ea poso: Sep-21-2023
